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ngc quest tm 10 plus chromatography system  (Bio-Rad)


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    Bio-Rad ngc quest tm 10 plus chromatography system
    Ngc Quest Tm 10 Plus Chromatography System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ngc+quest+tm+10+plus+chromatography+system/NGC+Quest+10+Plus+Chromatography+System/pmc12841349-188-22-29
    Average 96 stars, based on 207 article reviews
    ngc quest tm 10 plus chromatography system - by Bioz Stars, 2026-10
    96/100 stars

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    Purification:

    Article Title: Structural mechanisms behind the neutralisation of long-chain α-neurotoxins by broadly neutralising V H Hs discovered using a consensus antigen
    Article Snippet: .. For purification, the mixture was processed through an NGC Quest TM 10 Plus Chromatography System (Biorad) using a Superdex 75 10/300GL column (Cytiva) at 4 °C with the same buffer as the mobile phase. .. Before analysis, the concentration of the V H H:α-cobratoxin complex was increased to 15.2 mg/mL using 3.0 kDa MWCO Ultracentrifugation Units (UFC500324, Merck).

    Article Title: Preparation, Separation, and Identification of Low-Bitter ACE-Inhibitory Peptides from Sesame ( Sesamum indicum L.) Protein
    Article Snippet: .. The lyophilized DSPH-VI was dissolved in distilled water (50 mg/mL), then filtered through a 0.2 μm filter, and subsequently purified by an NGC Quest TM 10 Plus chromatography system (Bio-Rad Laboratories, Inc., Hercules, CA, USA) equipped with a C18 preparative column (20 mm × 250 mm I.D., 10 μm, Shimadzu, Kyoto, Japan). ..

    Chromatography:

    Article Title: Structural mechanisms behind the neutralisation of long-chain α-neurotoxins by broadly neutralising V H Hs discovered using a consensus antigen
    Article Snippet: .. For purification, the mixture was processed through an NGC Quest TM 10 Plus Chromatography System (Biorad) using a Superdex 75 10/300GL column (Cytiva) at 4 °C with the same buffer as the mobile phase. .. Before analysis, the concentration of the V H H:α-cobratoxin complex was increased to 15.2 mg/mL using 3.0 kDa MWCO Ultracentrifugation Units (UFC500324, Merck).

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Article Title: Preparation, Separation, and Identification of Low-Bitter ACE-Inhibitory Peptides from Sesame ( Sesamum indicum L.) Protein
    Article Snippet: .. The lyophilized DSPH-VI was dissolved in distilled water (50 mg/mL), then filtered through a 0.2 μm filter, and subsequently purified by an NGC Quest TM 10 Plus chromatography system (Bio-Rad Laboratories, Inc., Hercules, CA, USA) equipped with a C18 preparative column (20 mm × 250 mm I.D., 10 μm, Shimadzu, Kyoto, Japan). ..

    Transferring:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Blocking Assay:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Suction Filtration:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Imaging:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Cell Culture:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Hood:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Spectrophotometry:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Electrophoresis:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Flow Cytometry:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..

    Microscopy:

    Article Title: A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin
    Article Snippet: .. Micropipettes (Gilson, catalog number: F167360) Pipette aid (Drummond Scientific, catalog number: 4-000-102) Appropriate personal protective equipment 250 ml conical flask (Sigma, catalog number: CLS5100250) 2 L conical flask (Sigma, catalog number: CLS51002L) Magnetic stirring bar (Fisher Scientific, Fisherbrand TM , catalog number: 14-513-52) Magnetic stirrer (Thermolyne Nuova, catalog number: Z110930) Vortex mixer (Fisher Scientific, Vortex-Genie TM 2, catalog number: 15557335) Heat block (Benchmark Scientific, catalog number: BSW1500) Autoclave Tabletop incubator (Barnstead Lab Line 100) Centrifuge capable of spinning 500 ml bottles (Thermo Scientific, Sorvall Lynx 4000, catalog number: 75006580) 500 ml centrifuge bottles (Fisher Scientific, catalog number: 05-564-2) Emulsiflex C5 homogenizer (Avestin, catalog number: EF-C5) Tabletop microcentrifuge (Eppendorf, catalog number: 12843222) Platform Shaker at 4 °C (Orbital Shaker) Stericup-GP Vacuum Filtration System (Millipore, Millipore Sigma, catalog number: SCGPU05RE) NanoDrop 2000 (Thermo Scientific TM , catalog number: ND-2000) NGC Quest TM 10 Plus Chromatography system (Bio-Rad, catalog number: 7880003) Azure C300 gel imaging system (Azure biosystem) Cell culture incubator (Thermo Fisher Scientific, Forma TM Series II, catalog number: 3110) Biosafety cabinet (Labcult) Water bath Spectrophotometer pH meter Electrophoresis chamber (Bio-Rad, Mini-PROTEAN tetra) Powerpack (Bio-Rad, Powerpac TM universal power supply, catalog number: 1645070) -20 °C freezer -80 °C freezer BD LSRFortessa TM Flow cytometer system (BD biosciences) Spinning disk confocal head coupled to an inverted Zeiss Axiovert 200M microscope ..



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